Our established NGS analysis pipelines for DNA or RNA sequencing (Whole Genome Sequencing, WGS, Whole Exome Sequencing, WES, Transcriptome) offer various different possibilities and can be adapted to your specifications. This allows a high flexibility for meeting your requirements. We also process data from Xenograft material. All phases of the analysis process are subject to strict quality controls. The results are processed and presented in a clear and understandable manner in our web-based customer portal where they are available for download.
FASTQ raw data | BAM files | De-Novo Assemblies (FASTA)
Raw data in FASTQ format per sample, demultiplexed and pre-filtered. Files in BAM format: Mapping of reads to available reference genomes or transcriptomes. Binary Alignment Map (BAM). De-Novo-Assembly in FASTA format: If a reference is not available, we create an assembly from your sequence data as a reference for your genome or transcriptome. An assembly consists of so-called contigs which can be annotated using homologous genomes, if available.
DNA-seq | RNA-seq | ChIP-seq
Genome analyses, DNA seq: Whole Genome Sequencing or Whole Exome Sequencing: Analysis of structural variants, SNPs, smaller insertions, deletions and copy number variations (CNV). The results are provided as a VCF file (variant call format) and as extensively annotated tables.
Transcriptome analyses, RNA-seq: The analysis of differentially expressed genes or transcripts enables the discovery of new splice variants and isoforms. We provide statistics and lists with corresponding read numbers. It is also possible to analyse structural variants, SNPs, smaller insertions and deletions. As an additional service, we offer to generate lists of detected gene-fusion events.
Epigenetic analysis, ChIP-seq: Identification and annotation of significant peaks for the determination of protein-DNA interactions, e.g. binding sites of transcription factors, analysis of histone modifications and sequence motives.