The sequencing of complete genomes, Whole Genome Sequencing, yields comprehensive insights into all genetic features of an organism. In particular, this analysis also covers the non-protein coding regions outside the exome.
The exome represents less than 2 % of the human genome, but contains ~85 % of the known disease-causing genetic variants in the protein-coding regions. Potential applications are population studies, cancer studies and the investigation of genetic diseases. Exome sequencing is therefore the cost-effective alternative to genome sequencing (Whole Genome Sequencing, WGS).
ATLAS Biolabs offers targeted resequencing as a cost-effective alternative to genome and exome sequencing (WGS or WES) for the detection of genetic variants in customer-specific genomic target regions. We will develop the respective design in close consultation with our customer.
Chromatin immunoprecipitation (ChIP) sequencing enables the accurate analysis of interactions between proteins and DNA or RNA. ChIP sequencing thus provides the basis for the interpretation of essential, central regulatory mechanisms of many biological processes and diseases.
The RNA-Seq (RNA sequencing) technology allows the exploration and profiling of the transcriptome in any organism. Using RNA-Seq, it is possible to discover new transcripts, gene fusions, single nucleotide variants, InDels (small insertions and deletions) and other previously unknown changes. Small RNA-Seq can be used to analyse any small RNA or miRNA in any sample, including variations and differential expression profiles. In addition, new miRNAs and other small non-coding RNAs can be detected.
Transcriptome analyses at the gene and exon levels as well as for coding and non-coding regions.
Genotyping microarrays from Life Technologies (Affymetrix), Agilent and Illumina are used for genome-wide association studies (GWAS) and allow the accurate detection of point mutations / single nucleotide polymorphisms (SNP), copy number variations and chromosomal aberrations. This technology can be used to investigate the diversity of a species, disease-causing mutations or genetic traits associated with cancer, among other things. Besides human DNA samples, animal and plant samples can also be genotyped. These microarrays are also used in cytogenetics via molecular karyotyping. For its genotyping services, ATLAS Biolabs uses microarrays from Life Technologies (Affymetrix) on a wide range of samples (blood, cheek swab, saliva, cell culture, freshly frozen tissue, FFPE tissue), working even with the tiniest input samples.
Areas of Application:
The microarray-based analysis of expression profiles provides comprehensive results on gene activity in biological samples of various species. This cost-effective high-throughput method is able to yield high-quality data within a short time when conducting large studies on differential gene expression. Additional applications include disease classification, pathway analysis, mapping of expression-based quantitative trait loci (eQTL) and many more. Even the tiniest input samples, as in the case of FFPE samples, can be analysed in our laboratories without any problems.
The gene expression services of ATLAS Biolabs, certified by Life Technologies (Affymetrix) or Agilent, respectively, include microarrays of humans, mice and rats as well as many other microarrays for model organisms. Using special Affymetrix assays, it is possible to obtain results comparable to RNA-Seq in a manner that is fast, sensitive and validated. These assays allow the analysis of the differential exon-specific gene expression of the entire transcriptome and hence the analysis of many thousands of different transcript isoforms. Agilent custom microarrays are available in quantities starting with a single specimen slide. Upon request, we will also handle your custom design or support you when creating the design in Agilent's eArray.