NimbleGen Sequence Capture Service

• Up to 30 Mb of target sequence can be captured
• Empirically tested and proven capture design algorithm ensures a high level of specificity and sensitivity
• Processing of single samples is possible
• Built-in control probes allow for assessment of enrichment factor by quantitative PCR

• ATLAS Biolabs covers the complete process from array design to the generation of enriched samples. The customer just needs to send genomic coordinates, RefSeq IDs, or Gene IDs, in order to start the design process. Design and library generation are adapted to the sequencing platform preferred by the customer.
• If requested, ATLAS Biolabs will also perform Next-Generation Sequencing of the enriched sample.

ATLAS Biolabs is certified according to the international standards ISO 9001:2008 and ISO 13485. Our production processes are subject to rigorous quality control at all steps:

• Quality and quantity of each DNA sample sent to ATLAS Biolabs will be determined by agarose gel analysis and by photometric analysis. Samples which do not pass QC will not be processed.
• Numerous control steps are executed during library preparation, e.g. QC of successful DNA fragmentation, QC of successful linker ligation, etc.
• Enrichment of the genomic sequences of interest is assessed by quantitative PCR of control loci.
• All quality assurance procedures are electronically documented and available for our customers on demand.

• The customer will receive a sufficient amount of enriched DNA to perform at least 10 next-generation sequencing runs.
• The customer will be provided with a documentation of successful enrichment.

• Array design: 1 week
• Array production: 3 weeks
• Sequence enrichment: 2–3 weeks